Peroxidatic activity of liver microsomal cytochromeP-450
نویسندگان
چکیده
منابع مشابه
Purified Liver Microsomal NADPH-Cytochrome P-450 Reductase
NADPH-cytochrome P-450 reductase was isolated from liver microsomes of phenobarbital-induced rats. The enzyme exhibits an apparent minimal molecular weight of 76,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and contains 1 molecule each of FMN and FAD. Trypsin treatment of the reductase yields an enzyme with an apparent minimal molecular weight of 69,000 which r...
متن کاملPurified Liver Microsomal NADPH-Cytochrome P-450 Reductase
NADPH-cytochrome P-450 reductase was isolated from liver microsomes of phenobarbital-induced rats. The enzyme exhibits an apparent minimal molecular weight of 76,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and contains 1 molecule each of FMN and FAD. Trypsin treatment of the reductase yields an enzyme with an apparent minimal molecular weight of 69,000 which r...
متن کاملInteraction of liver microsomal cytochrome P-450 and NADPH-cytochrome P-450 reductase in the presence and absence of lipid.
Phospholipid has been reported to be necessary for optimal catalytic activity of a number of mammalian cytochrome P-450 (P-450) systems. We also confirm that a number of individual phospholipids and mixtures, used as soluble monomers or phospholipid vesicles, show activation of 7-ethoxycoumarin O-deethylase activity by an enzyme system composed of rat liver microsomal P-450PB-B and NADPH-P-450 ...
متن کاملIdentification of the high and low potential flavins of liver microsomal NADPH-cytochrome P-450 reductase.
NADPH-cytochrome P-450 reductase, which catalyzes electron transfer to cytochrome P-450 in liver microsomes in reactions leading to the hydroxylation of a variety of substrates, is known to contain 1 molecule each of FMN and FAD per polypeptide chain. The spectrophotometric changes accompanying stepwise reduction of the oxidized enzyme under anaerobic conditions have recently been described. In...
متن کاملProperties of partially purified liver microsomal cytochrome P-450: acceptance of two electrons during anaerobic titration.
A large variety of foreign compounds, including drugs, petroleum products and insecticides, as well as physiological substrates such as fatty acids and steroids, are attacked by molecular oxygen in the presence of NADPH, a reductase and liver microsomal cytochrome P-450 (P-450LM) as the oxygenating catalyst. Although much has been learned about the hydroxylation reactions catalyzed by liver mic...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1976
ISSN: 0014-5793
DOI: 10.1016/0014-5793(76)80958-1